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penetrans on potato in North America ( Brown et al. thornei, are commonly found coexisting with P. However, other Pratylenchus species, namely P. penetrans prior to planting essential for developing effective integrated pest control measures and making yield-loss risk assessment. This makes accurate identification and quantification of P. Seinhorst (1998) established that a significant correlation exists between the preplanting population numbers and the amount of damage caused by plant-parasitic nematodes. Reported yield reductions range from very slight to 30 to 50% ( MacGuidwin and Rouse 1990 Martin et al. This disease can lead to premature vine death and significant reduction in tuber size and total marketable yield and therefore can become a limiting factor in potato production. Yield losses may be exacerbated by interaction with the fungus Verticillium dahliae Kleb, causing a disease known as potato early dying disease complex (PED) ( MacGuidwin and Rouse 1990 Martin et al. The nematode infects potato tubers and causes a scabby appearance with sunken lesions or dark, wart-like bumps that turn purple on tubers in storage. 1987 MacGuidwin and Rouse 1990 Martin et al. In the U.S.A., the nematode is a major threat to many crops, especially potato growing in the northeastern part of the country ( Francl et al. The nematode is also recorded on different crops in Japan, Canada, Algeria and Morocco ( Mokrini et al. penetrans is known to be damaging on field crops, vegetables, and ornamental plants ( Coolen and D’Herde 1970 Pudasaini et al. It has a worldwide distribution but is commonly found in temperate regions on a variety of crops. The root-lesion nematode, Pratylenchus penetrans is one of the most economically damaging species within the genus Pratylenchus. The assay requires no expertise in nematode taxonomy and morphology, and may serve as a useful diagnostic tool in research, diagnostic labs, and extension services for pest management. penetrans from mixed populations of Pratylenchus spp., but also for efficient detection and quantification of P. The qPCR assay will not only be useful for differentiating P. Grinding the field soil prior to DNA extraction improved P. penetrans numbers artificially added to soil or estimated from naturally infested field soils by conventional methods, and the numbers quantified using the qPCR assay. There was a high correlation between the P. A standard curve relating threshold cycle and log values of nematode number was generated from artificially infested soils. penetrans populations and 31 isolates of other nematode species. The specificity of the assay was evaluated using eight isolates of P. penetrans-specific qPCR primers were designed from the D2-D3 region of the 28S rDNA. A SYBR Green I-based qPCR assay was developed to discriminate, identify, and quantify P. penetrans prior to planting are essential for developing effective integrated pest control measures. Accurate identification and quantification of P. Yield losses may be exacerbated by interaction with the fungus Verticillium dahliae in the potato early dying disease complex. The root-lesion nematode Pratylenchus penetrans is a major pathogen of potato worldwide.